PERMEABILITY OF HYDROPHILIC MODEL DRUGS MODEL DRUGS ACROSS 2/4/A1 INTESTINAL EPITHELIAL CELL MONOLAYERS MODEL DRUGS ACROSS 2/4/A1 INTESTINAL EPITHELIAL CELL MONOLAYERS

MODEL DRUGS ACROSS 2/4/A1 INTESTINAL epithelial CELL MONOLAYERS SUMMARY Investigations of the integrity and broadcast characteristics of 2/4/A1 cadres corroborate been done in this report. The cell patronage was uninvolved from rat fetal enteric epithelial cells and transfected with thermolabile SV40 stupendous T antigen. These cells proliferated at 33 °C, unless eliminated the antigen and ceased proliferating at a non-permissive temperature (39°C). At 39°C 2/4/A1 cells started to differentiate entirely simultaneously the cells also underwent monumental cell death. When cultured at 37°C these cells create confluent and implike monolayers that seemed to have paracellular transport characteristics similar to that of the mankind intestine. Transmission electron microscopy sustain the development of multilayers at 33°C, monolayers at 37°C and defects in the cell layer collect to apoptosis at 39°C. Different immunostainings of ZO-1, E-cadherin an d vinculin confirmed formation of tight and union junctions. Transepithelial resistance reached a plateau of 25-35 Ohm.cm2, which was similar to the minuscular intestine. In transport studies 2/4/A1 cell line monolayers selectively restricted the permeation of hydrophilic permeability markers proportionate to molecular(a)(a) weight and discriminated more accurately between the molecules of mediocre molecular weight compared to Caco-2 cells. These results indicated that 2/4/A1 cells could be utilize as a model for hydrophilic drug engrossment.

INTRODUCTION The excellent intestine plays a cruc ial role in the absorption of drugs and nut! rients. Exogenous substances cross a series of barriers during the process of intestinal absorption: (1) the aqueous boundary/mucus layer, (2) a constitution layer of epithelial cells, and (3) the lamina propria, which contains the blood and lymph vessels that then transport the apparel drugs to other parts of the body (Artursson 1991). The cell monolayer is comprised of two number barriers: the cell membrane and the tight junctions. Most drugs are disengage up by a passive diffusion across the cell membrane... If you want to get a full essay, modulate it on our website: OrderCustomPaper.com

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